Recombinant Human MCM3 protein (His tag)

Specifications

Catalog number

PDEH100799-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with mCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Abbreviation

MCM3

Synonyms

MCM 3; MCM3; P1 Protein; P1-MCM3; P1.h; P102 protein; mcm3; p102

UniProt

P25205

Accession Number

P25205

Expression System

E.coli

Tag

N-His & C-His

Sequence

Ser 41-Ala 300

Field of Research

Cancer; Epigenetics and Nuclear Signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.5 kDa

Observed Molecular Weight

35 kDa

Species

Human