Recombinant Human ADA protein (His tag)

Specifications

Catalog number

PDEH100812-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adenosine Desaminase (ADA) deficiency, is a purine metabolic disorder that cause severe combined immunodeficiency (SCID) due to the accumulation of toxic metabolites that primarily affects development, differentiation and function of T and B lymphocytes. Adenosine deaminase is a polymorphic enzyme that has an important role in immune functions and in the regulation of intracellular and extracellular concentrations of adenosine and adenosine receptor activity. ADA activity might be considered as a useful diagnostic tool among the other markers in these diseases. Genetic variability of ADA activity may have, therefore, an important role in resistance to malaria. Adenosine Deaminase (ADA) deficiency is an autosomal recessive variant of severe combined immunodeficiency (SCID) caused by systemic accumulation of ADA substrates.

Abbreviation

ADA

Synonyms

ADA; ADA1; Adenosine Deaminase; Adenosine aminohydrolase; EC 3.5.4.4; adenine deaminase

UniProt

P00813

Accession Number

P00813

Expression System

E.coli

Tag

N-His

Sequence

Lys 11-Val 280

Field of Research

Cancer; Epigenetics and Nuclear Signaling; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.6 kDa

Observed Molecular Weight

32 kDa

Species

Human