Recombinant Human Collagen α-1 (III) Chain/COL3A1 protein (His tag)

Specifications

Catalog number

PDEH100820-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Abbreviation

Collagen α-1 Chain; COL3A1

Synonyms

CO3A1; CO3A1_HUMAN; Collagen alpha-1 (III) chain; PIIICP

UniProt

P02461

Accession Number

P02461

Expression System

E.coli

Tag

N-His

Sequence

Asp 1222-Leu 1466

Field of Research

Signal Transduction; Cell biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26.8 kDa

Observed Molecular Weight

35 kDa

Species

Human