Recombinant Human TAFI protein (His tag)

Specifications

Catalog number

PDEH100823-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carboxypeptidase B2 (CPB2) is a secreted enzyme that belongs to the peptidase M14 family. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen by the liver and circulates in the plasma as a plasminogen-bound zymogen. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides, such as kinins or anaphylatoxins, in the circulation regulating their activities. CPB2 also down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. CPB2 exhibits carboxypeptidase activity when it is activated by proteolysis at residue Arg92 of the thrombin/thrombomodulin complex. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Abbreviation

TAFI

Synonyms

CPB2; CPU; Carboxypeptidase B2; Carboxypeptidase U; Plasma Carboxypeptidase B; TAFI; Thrombin-Activable Fibrinolysis Inhibitor; pCPB

UniProt

Q96IY4

Accession Number

Q96IY4

Expression System

E.coli

Tag

N-His

Sequence

Glu 138-Val 386

Field of Research

Signal Transduction; Cardiovascular; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.3 kDa

Observed Molecular Weight

32 kDa

Species

Human