Recombinant Human IL-13 protein (SUMO, His tag)

Specifications

Catalog number

PDEH100844-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4R&alpha, ) and at least one of two known IL-13-specific binding chains (IL-13 R&alpha,1 and IL-13 R&alpha,2) . As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.

Abbreviation

IL-13

Synonyms

BHR; IL13; IL-13; P600; ALRH; BHR1; NC30; Interleukin-13

UniProt

P35225

Accession Number

P35225

Expression System

E.coli

Tag

N-SUMO-His

Sequence

Leu 25-Asn 146

Field of Research

Cancer; Cardiovascular; Immunology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.3 kDa

Observed Molecular Weight

35 kDa

Species

Human