Recombinant Human INHB protein (His tag)

Specifications

Catalog number

PDEH100864-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to one another by a single disulfide bond. Activin is composed of two ß subunits, ßA ßA (activin A), ßB ßB (activin B), or ßA ßB (activin AB) . Inhibin is composed of an alpha and one of two ß subunits, ßA (inhibin A) or ßB (inhibin B) . Activins are produced in many cell types and organs, such as gonads, pituitary gland, and placenta. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. Also, Activin plays a role in wound repair and skin morphogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in the epidermis of transgenic mice improves wound healing and enhances scar formation. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and kidney. There is also evidence showed that lack of activin during development results in neural developmental defects.

Abbreviation

INHB

Synonyms

Activin beta-B chain; INHA; INHB; INHBB

UniProt

P05111; P09529

Accession Number

P05111&P09529

Expression System

E.coli

Tag

N-His

Sequence

P05111 (Ser 233–Ile 366) -GGGGS*3-P09529 (Gly 293–Ala 407)

Field of Research

Signal Transduction; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.2 kDa

Observed Molecular Weight

31 kDa

Species

Human