Recombinant Human PODXL protein (SUMO, His tag)

Specifications

Catalog number

PDEH100865-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family.Podocalyxin is expressed on glomerular and vascular endothelial cells, neurons, hematopoietic, mesenchymal, and cardiac stem cells, and metastatic carcinoma and glioblastoma tumor cells. A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia. Podocalyxin promotes platelet activation, neurite outgrowth, branching, and synaptogenesis, homing of hematopoietic progenitor cells to the bone marrow and spleen, and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney. Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium.

Abbreviation

PODXL

Synonyms

PCLP; PCLP-1; PCLP1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; PODXL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like

UniProt

O00592

Accession Number

O00592

Expression System

E.coli

Tag

N-SUMO-His

Sequence

Ala 220-Ile 431

Field of Research

Signal Transduction; Stem Cells; Cancer; Cardiovascular

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.2 kDa

Observed Molecular Weight

32 kDa

Species

Human