Recombinant Human RANTES protein (GST&His tag)

Specifications

Catalog number

PDEH100876-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Chemokine (C-C Motif) Ligand 5 (CCL5) plays an active role in recruiting leukocytes into inflammatory sites. CCL5 is secreted by many cell types at inflammatory sites and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. N-Terminal truncated CCL5/RANTES, Met-RANTES, and amino-oxypentane (AOP) -RANTES exhibit antagonist or partial agonist functions on their receptors. CCL5/RANTES attracts different subtypes of leukocytes into inflamed tissue and intervenes in a wide range of allergic and autoimmune diseases.

Abbreviation

RANTES

Synonyms

C-C Motif Chemokine 5; CCL5; D17S136E; EoCP; Eosinophil Chemotactic Cytokine; SCYA5; SIS-Delta; Small-Inducible Cytokine A5; T Cell-Specific Protein P228; T-Cell-Specific Protein RANTES; TCP228

UniProt

P13501

Accession Number

P13501

Expression System

E.coli

Tag

N-GST & C-His

Sequence

Pro 25-Ser 91

Field of Research

Cardiovascular; immunology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.3 kDa

Observed Molecular Weight

42 kDa

Species

Human