Recombinant Human Synuclein-α (SNCA) protein (His tag)

Specifications

Catalog number

PDEH100877-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N-and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD) . In addition, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.

Abbreviation

Synuclein-α

Synonyms

CD309; FLK-1; FLK1; Fetal liver kinase 1; Flk-1; KDR; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; VEGFR; VEGFR-2; VEGFR2; Vascular endothelial growth factor receptor 2

UniProt

P37840

Accession Number

P37840

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Ala 140

Field of Research

Cancer; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

15.3 kDa

Observed Molecular Weight

19 kDa

Species

Human