Recombinant Human CXCL11 protein (His tag)

Specifications

Catalog number

PDEH100884-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CXCL11, also known as I-TAC, SCYB9B, H174 and beta-R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

Abbreviation

CXCL11

Synonyms

B-R1; CXCL11; I-TAC; ITAC; Interferon Inducible T-Cell α Chemokine; SCYB11; SCYB9B

UniProt

O14625

Accession Number

O14625

Expression System

E.coli

Tag

C-His

Sequence

Phe 22-Phe 94

Field of Research

Cancer; Immunology; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

7.9 kDa

Observed Molecular Weight

12 kDa

Species

Human