Recombinant Human Nectin-4/NECTIN4 protein (His tag)

Specifications

Catalog number

PDEH100892-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and-heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

Abbreviation

Nectin-4; NECTIN4

Synonyms

Ig superfamily receptor LNIR; LNIR; Nectin-4; PRR4; PVRL4; Poliovirus receptor-related protein 4

UniProt

Q96NY8

Accession Number

Q96NY8

Expression System

E.coli

Tag

N-His

Sequence

Gly 32-Leu 146

Field of Research

Cell biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.5 kDa

Observed Molecular Weight

15 kDa

Species

Human