Recombinant Human ER alpha protein (His tag)

Specifications

Catalog number

PDEH100893-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Estrogen Receptor is a major ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily. Estrogen Receptor is composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but they also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Abbreviation

ER alpha

Synonyms

ER; ER-Alpha; ESR; ESR1; Estradiol Receptor; Estrogen Receptor; Estrogen Receptor α; NR3A1; Nuclear Receptor Subfamily 3 Group A Member 1

UniProt

P03372

Accession Number

P03372

Expression System

E.coli

Tag

N-His

Sequence

Met 297-Ser 554

Field of Research

Signal Transduction; Neuroscience; Cancer; epigenetics and nuclear signal

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.3 kDa

Observed Molecular Weight

31 kDa

Species

Human