Recombinant Human TIMP3 protein (His tag)

Specifications

Catalog number

PDEH100896-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Abbreviation

TIMP3

Synonyms

HSMRK222; K222TA2; MIG 5 protein; Metalloproteinase inhibitor 3; SFD; TIMP 3; TIMP-3; Tissue Inhibitor of Metalloproteinase 3

UniProt

P35625

Accession Number

P35625

Expression System

E.coli

Tag

N-His

Sequence

Cys 24-Asn 144

Field of Research

Signal Transduction; Cardiovascular; Cancer; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.2 kDa

Observed Molecular Weight

15 kDa

Species

Human