Recombinant Human SMOC2 protein (His tag)

Specifications

Catalog number

PDEH100900-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins. The human SMOC-2 cDNA encodes 446 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40-84), two thyroglobulin type-1 segments (aa 87-153 and 213-281) and two EF-hand sequences (aa 347-382 and 384-419) . Of three splice variants, one shows a 13 aa substitution for aa 442-446, another shows an 11 aa insertion after Thr170, and a third contains both of these variations. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions. Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6. SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1. When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis. SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle. In the skin, it promotes keratinocyte attachment and migration. Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery.

Abbreviation

SMOC2

Synonyms

MST117; MSTP117; MSTP140; SMAP 2; SMAP-2; Secreted modular calcium binding protein 2; Smooth muscle associated protein 2

UniProt

Q9H3U7

Accession Number

Q9H3U7

Expression System

E.coli

Tag

N-His

Sequence

Gln 229-Val 415

Field of Research

Signal Transduction; Cardiovascular

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.5 kDa

Observed Molecular Weight

32 kDa

Species

Human