Recombinant Human NANOG protein (His tag)

Specifications

Catalog number

PDEH100904-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity) . Acts as a transcriptional activator or repressor (By similarity) . Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity) . When overexpressed, promotes cells to enter into S phase and proliferation.

Abbreviation

NANOG

Synonyms

ENK; Embryonic stem cell specific homeobox protein; FLJ12581; Homeobox protein NANOG; hNanog

UniProt

Q9H9S0

Accession Number

Q9H9S0

Expression System

E.coli

Tag

N-His

Sequence

Lys 94-Ser 162

Field of Research

Stem Cell; Epigenetics and Nuclear Signaling; Cancer; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

7.5 kDa

Observed Molecular Weight

12 kDa

Species

Human