Recombinant Human ACTA2 protein (His tag)

Specifications

Catalog number

PDEH100905-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β-and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells.

Abbreviation

ACTA2

Synonyms

AAT6; ACTSA; Actin aortic smooth muscle; GIG46; Growth inhibiting gene 46; MYMY5; a actin

UniProt

P62736

Accession Number

P62736

Expression System

E.coli

Tag

N-His

Sequence

Cys 2-Phe 377

Field of Research

Cardiovascular; Signal Transduction; Stem Cell; Developmental Biology ImmunologySignal Transduction; Stem Cell; Cardiovascular; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.3 kDa

Observed Molecular Weight

42 kDa

Species

Human