Recombinant Human PTPN11 protein (His tag)

Specifications

Catalog number

PDEH100916-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP) . It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines. In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 at p85 binding sites. SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy terminus in response to growth factor receptor activation. These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity. Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation.

Abbreviation

PTPN11

Synonyms

BPTP3; CFC; JMML; METCDS; NS1; PTN11; PTP-1D; PTP2C; SAP2; SH-PTP2; SH-PTP3; SHP2; SHPTP2; SHPTP3; Syp

UniProt

Q06124

Accession Number

Q06124

Expression System

E.coli

Tag

N-His

Sequence

Thr 2-Ala 521

Field of Research

Signal Transduction; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.1 kDa

Observed Molecular Weight

60 kDa

Species

Human