Recombinant Human Cyclin A2/CCNA2 protein (His tag)

Specifications

Catalog number

PDEH100921-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

Abbreviation

Cyclin A2; CCNA2

Synonyms

CCN1; Cyclin-A2

UniProt

P20248

Accession Number

P20248

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Leu 432

Field of Research

Cardiovascular; Cancer; Epigenetics and Nuclear Signaling; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

47.4 kDa

Observed Molecular Weight

55 kDa

Species

Human