Recombinant Human cyclin D1/Ccnd1 protein (His tag)

Specifications

Catalog number

PDEH100922-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation.

Abbreviation

Cyclin D1; Ccnd1

Synonyms

B cell leukemia 1; BCL 1; Cd1; Cyl 1; G1/S specific cyclin D1; PRAD1; ccnd1

UniProt

P25322

Accession Number

P25322

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ile 295

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.3 kDa

Observed Molecular Weight

39 kDa

Species

Human