Recombinant Human CD62L/L-Selectin protein (His tag)

Specifications

Catalog number

PDEH100949-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naïve and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.

Abbreviation

CD62L; L-Selectin

Synonyms

CD62 antigen-like family member L; CD62L; L-selectin; Leukocyte adhesion molecule 1 (LAM-1) ; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; Lymphocyte antigen 22 (Ly-22) ; Lymphocyte surface MEL-14 antigen; Sell

UniProt

P30836

Accession Number

P30836

Expression System

E.coli

Tag

N-His

Sequence

Trp 39-Asn 332

Field of Research

Stem Cells; Cardiovascular; Immunology; signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.2 kDa

Observed Molecular Weight

39 kDa

Species

Human