Recombinant Human LDHB protein (His tag)

Specifications

Catalog number

PDEH100952-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

L-Lactate Dehydrogenase B Chain (LDH-B) is a member of the lactate dehydrogenase family that consists of three members, LDH-A, LDH-B and LDH-C, members of this family function as powerful markers for germ cell tumors. LDH-B is an oxidoreductase that catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate to lactate when oxygen is absent or in short supply and it performs the reverse reaction during the Cori cycle in the liver. It is also called Hydroxybutyrate Dehydrogenase (HBD) due to its ability to catalyze the oxidation of hydroxybutyrate.

Abbreviation

LDHB

Synonyms

L-lactate Dehydrogenase B Chain; LDH Heart Subunit; LDH-B; LDH-H; LDHB; Renal Carcinoma Antigen NY-REN-46

UniProt

P07195

Accession Number

P07195

Expression System

E.coli

Tag

N-His

Sequence

Ala 2-Leu334

Field of Research

Cancer; Metabolism; Signal Transduction; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.5 kDa

Observed Molecular Weight

40 kDa

Species

Human