Recombinant Human CD147/BSG/Basigin protein (His tag)

Specifications

Catalog number

PDEH100954-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

Abbreviation

CD147; BSG; Basigin

Synonyms

5F7; BSG; Basigin; CD147; Collagenase Stimulatory Factor; EMMPRIN; Extracellular Matrix Metalloproteinase Inducer; Leukocyte Activation Antigen M6; OK Blood Group Antigen; TCSF; Tumor Cell-Derived Collagenase Stimulatory Factor

UniProt

P35613

Accession Number

P35613

Expression System

E.coli

Tag

N-His

Sequence

Glu 138-Ala 323

Field of Research

Cardiovascular; Neuroscience; Cancer; immunology; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.4 kDa

Observed Molecular Weight

25 kDa

Species

Human