Recombinant Human CREB3L4 protein (His tag)

Specifications

Catalog number

PDEH100966-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The cyclic AMP responsive element-binding protein 3-like 4 (CREB3L4) is a transcription factor highly expressed in multiple human cancers. This study aimed to investigate the regulatory effects of CREB3L4 on GC progression and angiogenesis. CREB3L4 was overexpressed in GC tissues and cell lines, and was positively correlated with advanced tumor stage and poor survival in GC patients. The upregulation of CREB3L4 in GC cells increased cell viability, promoted cell proliferation, reduced apoptosis, enhanced cell migration and invasion, and induced the formation of tubule-like endothelial structures, whereas CREB3L4 knockdown impeded tumor cell growth, attenuated cell motility, and prevented human umbilical vein endothelial cells from forming tubule-like structures. In addition, mice inoculated with CREB3L4-deficient GC cells showed significantly suppressed tumor growth compared to the group harboring wild-type tumors.

Abbreviation

CREB3L4

Synonyms

Androgen-induced basic leucine zipper protein (AIbZIP) ; Attaching to CRE-like 1 (ATCE1) ; CREB3L4; Cyclic AMP-responsive element-binding pr; Cyclic AMP-responsive element-binding protein 3-like protein 4; cAMP-responsive element-binding protein 3-like protein 4

UniProt

Q8TEY5

Accession Number

Q8TEY5

Expression System

E.coli

Tag

N-His & C-His

Sequence

Glu 14-Ser 295

Field of Research

Epigenetics and Nuclear signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.2 kDa

Observed Molecular Weight

40 kDa

Species

Human