Recombinant Human Vinculin/VCL protein (His tag)

Specifications

Catalog number

PDEH100977-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/α-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction (s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.

Abbreviation

Vinculin; VCL

Synonyms

CMD1W; CMH15; HEL114; MV; MVCL; Metavinculin; VCL; Vinculin

UniProt

P18206

Accession Number

P18206

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Val650

Field of Research

Cardiovascular; signal Transduction; Epigenetics and Nuclear signaling; Isotype/Loading Controls

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

71.4 kDa

Observed Molecular Weight

60 kDa

Species

Human