Recombinant Human Sperm-associated antigen 5/SPAG5 protein (His tag)

Specifications

Catalog number

PDEH100978-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Astrin is a homodimeric protein with a globular head domain and a long stalk. It is an essential component of the mitotic spindle. Astrin is highly expressed in testis and is detected at low levels in placenta, liver, pancreas, thymus and colon. Its expression is cell cycle-regulated, with an increase from prophase to cytokinesis and return to basal levels at the next G1 phase. In non-dividing cells Astrin is associated with microtubules unless under cellular stress, then it is localized in stress granules.

Abbreviation

SPAG5

Synonyms

Astrin; Deepest; Mitotic spindle-associated protein p126; SPAG5; Sperm-associated antigen 5

UniProt

Q96R06

Accession Number

Q96R06

Expression System

E.coli

Tag

N-His & C-His

Sequence

Asn 482-Ala 850

Field of Research

Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.5 kDa

Observed Molecular Weight

48 kDa

Species

Human