Recombinant Human SEC13 protein (His tag)

Specifications

Catalog number

PDEH100985-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport.

Abbreviation

SEC13

Synonyms

GATOR complex protein SEC13; Protein SEC13 homolog; SEC13; SEC13-like protein 1; SEC13-related protein

UniProt

P55735

Accession Number

P55735

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Gln 322

Field of Research

Signal Transduction; Tags and Cell Markers

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.3 kDa

Observed Molecular Weight

40 kDa

Species

Human