Recombinant Human PPA1 protein (His tag)

Specifications

Catalog number

PDEH101009-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PPA1 (Inorganic pyrophosphatase 1, also PPase and IOPPP) is a 32-36 kDa cytoplasmic member of the PPase family of enzymes. It is ubiquitously expressed, and acts on di- (or pyro) phosphate, generating orthophosphate in a Mg+-dependent manner. This activity can both generate energy for cells, or in the case of osteoblasts, provide raw material for calcification. The consumption of pyrophosphate may also remove inhibitors of enzymes such as guanylyl cyclase, and PPA1 itself is also reported to stimulate gene expression. Human PPA1 is 289 amino acids (aa) in length. There is one pyrophosphatase domain between aa 42-255, and two utilized acetylation sites at Lys 57 and Lys228. PPA1 is known to form homodimers. There is one potential alternative start site at Met46. Full-length human PPA1 shares 94% aa sequence identity with mouse PPA1.

Abbreviation

PPA1

Synonyms

Inorganic pyrophosphatase; PPA1; Pyrophosphate phospho-hydrolase (PPase)

UniProt

Q15181

Accession Number

Q15181

Expression System

E.coli

Tag

N-His & C-His

Sequence

Ser 2-Asn 289

Field of Research

Metabolism; signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

31.6 kDa

Observed Molecular Weight

36 kDa

Species

Human