Recombinant Human PP2A-Cα/PPP2CA protein (His tag)

Specifications

Catalog number

PDEH101010-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits, a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab) . PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.

Abbreviation

PP2A-Cα; PPP2CA

Synonyms

PP2A-alpha; PPP2CA; Replication protein C (RP-C) ; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

UniProt

P67775

Accession Number

P67775

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Leu 309

Field of Research

Cancer; Signal Transduction; Cell Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.3 kDa

Observed Molecular Weight

40 kDa

Species

Human