Recombinant Human PGD protein (His tag)

Specifications

Catalog number

PDEH101015-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

6-phosphogluconate dehydrogenase (PGD) is a cytoplasm-located protein, and belongs to the 6-phosphogluconate dehydrogenase family. 6PGD is the second dehydrogenase in the pentose phosphate shunt. It catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH. Mutations within the gene coding this enzyme result in 6-phosphogluconate dehydrogenase deficiency, an autosomal hereditary disease effecting the red blood cells.

Abbreviation

PGD

Synonyms

6-phosphogluconate dehydrogenase;6PGD; Decarboxylating; PGD; PGDH

UniProt

P52209

Accession Number

P52209

Expression System

E.coli

Tag

N-His & C-His

Sequence

Met 1-Ala 483

Field of Research

Signal Transduction; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.0 kDa

Observed Molecular Weight

55 kDa

Species

Human