Recombinant Human MAPK12 protein (His tag)

Specifications

Catalog number

PDEH101048-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ERK3, also known as MAPK12 and p38-gamma, belongs to the&nbsp, protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. ERK3 is highly expressed in skeletal muscle and heart.&nbsp, ERK3 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

Abbreviation

MAPK12

Synonyms

ERK-6; ERK3; ERK6; MAPK12; P38-gamma; P38GAMMA; PRKM12; SAPK-3; SAPK3

UniProt

P53778

Accession Number

P53778

Expression System

E.coli

Tag

N-His & C-His

Sequence

Asp 101-Leu 367

Field of Research

Signal Transduction; Cell biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.3 kDa

Observed Molecular Weight

40 kDa

Species

Human