Recombinant Human E2F2 protein (His tag)

Specifications

Catalog number

PDEH101062-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

E2F-2 (viral E2-associated factor 2) is a 50 55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA activating E2F heterodimeric complex with DP-1 or-2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F 2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65 105), a DNA binding domain (aa 107 196), a dimerization segment (aa 197 289), a transactivation region (aa 359 437), and a pRB binding domain (aa 410 427) . There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349 437. Over aa 308 437, human E2F-2 is 72% aa identical to mouse E2F-2.

Abbreviation

E2F2

Synonyms

E2F2; Transcription factor E2F2

UniProt

Q14209

Accession Number

Q14209

Expression System

E.coli

Tag

N-His

Sequence

Ala 65-Asn 437

Field of Research

Epigenetics and Nuclear signaling

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.9 kDa

Observed Molecular Weight

55 kDa

Species

Human