Recombinant Human OPG protein (GST, His tag)

Specifications

Catalog number

PDEH101068-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TNFRSF11B is a secreted protein, containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL) . By binding RANKL, TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/macrophages and are derived from granulocyte/macrophage-forming colony units (CFU-GM) ) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between

Abbreviation

OPG

Synonyms

OCIF; OPG; Osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; TNFRSF11B; TR1; Tumor necrosis factor receptor superfamily member 11B

UniProt

O00300

Accession Number

O00300

Expression System

E.coli

Tag

N-GST & C-His

Sequence

Glu 22-Glu 300

Field of Research

Signal Transduction; Cardiovascular; immunology; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

55.6 kDa

Observed Molecular Weight

55 kDa

Species

Human