Recombinant Human ABCG2 protein (His tag)

Specifications

Catalog number

PDEH101078-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) . Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects . An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line , is expressed on stem cells . ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates

Abbreviation

ABCG2

Synonyms

Broad substrate specificity ATP-binding cassette transporter ABCG2; ABCG2; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338

UniProt

Q9UNQ0

Accession Number

Q9UNQ0

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Gly 300

Field of Research

Cancer; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Biochemicals

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

32.9 kDa

Observed Molecular Weight

32 kDa

Species

Human