Recombinant Human PTCH1 protein (His tag)

Specifications

Catalog number

PDEH101084-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH) . Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

Abbreviation

PTCH1

Synonyms

PTC; PTC1; PTCH1; Protein patched homolog 1

UniProt

Q13635

Accession Number

Q13635

Expression System

E.coli

Tag

N-His

Sequence

Glu 122-Asp 436

Field of Research

Cancer; Cell Biology; Neuroscience; Stem Cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.5 kDa

Observed Molecular Weight

38 kDa

Species

Human