Recombinant Mouse Chromogranin-A/Chga protein (His Tag)

Specifications

Catalog number

PDEM100227-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines.Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation . Pyroglutaminated (pGlu) -serpinin exerts an antiapoptotic effect on cells exposed to oxidative stress .

Abbreviation

Chromogranin-A; Chga

Synonyms

CHG A; CMGA; CgA; Chga; Chromogranin A; Chromogranin A parathyroid secretory protein 1; Chromogranin A precursor; ChromograninA; ER-37; Pancreastatin; Parastatin; Parathyroid secr; beta Granin; betagranin (N-terminal fragment of chromogranin A) ; catestatin; chromofungin

UniProt

P26339

Accession Number

P26339

Expression System

E.coli

Tag

N-His

Sequence

Leu19-Met150

Applications

Immunogen

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.4 KDa

Observed Molecular Weight

15-20 kDa

Species

Mouse