Recombinant Mouse Galectin-3/Lgals3 protein (His Tag)

Specifications

Catalog number

PDEM100233-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Abbreviation

Galectin-3; Lgals3

Synonyms

35 kDa lectin; CBP35; Carbohydrate binding protein 35; GAL3; GALBP; GALIG; GBP; Gal-3; IgE binding protein; LGALS2; Lectin L-29; Mac-2 antigen; Macrophage galactose-specific lectin

UniProt

P16110

Accession Number

P16110

Expression System

E.coli

Tag

N-His

Sequence

Ala2-Ile264

Applications

Immunogen

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.4 kDa

Observed Molecular Weight

31-33 kDa

Species

Mouse