Recombinant Mouse AFP Protein (His Tag)

Specifications

Catalog number

PDEM100237-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Abbreviation

AFP

Synonyms

AFPD; Afp; Alpha fetoglobulin; Alpha fetoprotein; Alpha fetoprotein precursor; Alpha-1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein; FETA; HPAFP; Hereditary persistence of; Hereditary persistence of alpha fetoprotein; alpha-fetoprotein; included

UniProt

P02772

Accession Number

P02772-1

Expression System

E.coli

Tag

N-His

Sequence

Tyr273-Val605

Applications

Immunogen

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.9 kDa

Observed Molecular Weight

28.2 kDa

Species

Mouse