Recombinant Mouse Component C3a protein (TRX, His tag)

Specifications

Catalog number

PDEM100242-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complement is defined as key part of innate immunity and as the first line of defense in the fight against invading pathogens. Complement 3 (C3) is the most abundant component of the complement cascade and the convergent point for all three major complement activation pathways: namely classical, alternative and mannose-binding lectin pathways. Complement activation leads to the formation of the C3 convertase, which cleaves C3 into the key effector molecules, C3a (anaphylatoxin) and C3b (opsonin) which then drive microbe removal. By binding to C3a receptor (C3aR), C3a exhibits potent anaphylatoxin activity, including increased vascular permeability, triggering degranulation of mast cells, inflammation, and activating leukocytes.

Abbreviation

Component C3a

Synonyms

AHUS5; ARMD9; ASP; Acylation stimulating protein; C3; Complement C3; Complement component C

UniProt

P01027

Accession Number

P01027

Expression System

E.coli

Tag

N-Trx-His

Sequence

Ser 671-Arg 748

Field of Research

Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.5 kDa

Observed Molecular Weight

32 kDa

Species

Mouse