Recombinant Mouse ACTH protein (His tag)

Specifications

Catalog number

PDEM100257-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adrenocorticotropic hormone (ACTH) /corticotropin is a 39 amino acid peptide generated by cleavage of the POMC, a preprotein which also can also be cleaved to produce MSH, Beta-endorphin and Met-enkephalin, depending on tissue expression and context. ACTH is a ligand for the ACTH receptor, also known as melanocortin receptor 2. ACTH is secreted by the anterior pituitary gland upon stimulation by corticotropin-releasing hormone (CRH) which is secreted by the hypothalamus. The principal effect of ACTH is production of cortisol by the adrenal gland but it also mediates bone formation and osteoblast survival. ACTH has been shown to induce insulin resistance in adipocytes and promote pro-inflammatory adipokine secretion.

Abbreviation

ACTH

Synonyms

ACTH; Adrenocorticotropic hormone; Adrenocorticotropin; POC; POMC; Pmc2; Pomc; Pomc-1; Pomc1

UniProt

P01193

Accession Number

P01193

Expression System

E.coli

Tag

N-His

Sequence

Trp 27-Gln 235

Field of Research

Cancer; Metabolism; Neuroscience; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.9 kDa

Observed Molecular Weight

30 kDa

Species

Mouse