Recombinant Mouse IL-33 protein (His tag)

Specifications

Catalog number

PDEM100264-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.

Abbreviation

IL-33

Synonyms

DVS; NF-HEV and IL- 1F; Interleukin-1 family member; C9orf; C9orf26; DVS27; Interleukin-1 family member 11; NF-HEV and IL- 1F11; NKHEV; IL33; Interleukin-33; IL-33; Interleukin 33; IL1F11; NFEHEV; NF-HEV

UniProt

Q8BVZ5

Accession Number

Q8BVZ5

Expression System

E.coli

Tag

N-His

Sequence

Ser 109-Ile 266

Field of Research

Cardiovascular; Immunology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.3 kDa

Observed Molecular Weight

28 kDa

Species

Mouse