Recombinant Mouse IL1A/IL-1A/IL-1F1 protein (His tag)

Specifications

Catalog number

PDEM100275-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Abbreviation

IL1A; IL-1A; IL-1F1

Synonyms

Hematopoietin; IL1F; Hematopoietin-1; IL1; IL1-A; IL1F1; IL-1α; Preinterleukin 1 Alpha; Pro-Interleukin-1-Alpha; Il-1a; Interleukin-1 alpha; IL-1 alpha; Il1a

UniProt

P01582

Accession Number

P01582

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Ser 270

Field of Research

Immunology; Metabolism; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.6 kDa

Observed Molecular Weight

35 kDa

Species

Mouse