Recombinant Mouse HP1α protein (His tag)

Specifications

Catalog number

PDEM100278-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) . Can interact with lamin-B receptor (LBR) . This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Abbreviation

HP1α

Synonyms

Antigen p25; CBX5; Chromobox 5; Epididymis luminal protein 25; HEL25; HP1 alpha; HP1A; Heterochromatin protein 1

UniProt

Q61686

Accession Number

Q61686

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Ser 191

Field of Research

Epigenetics and Nuclear Signaling; Tags & Cell Markers

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.9 kDa

Observed Molecular Weight

25 kDa

Species

Mouse