Recombinant Mouse BCR protein (His tag)

Specifications

Catalog number

PDEM100283-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain, and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac. The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Research studies have shown that the Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) . Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl) . Phosphorylated Tyr177 provides a docking site for Gab2 and GRB2.

Abbreviation

BCR

Synonyms

ALL; Breakpoint cluster region; CML; D22S662; FGFR1/BCR chimera protein; PHLRenal carcinoma antigen NY-REN-26

UniProt

Q6PAJ1

Accession Number

Q6PAJ1

Expression System

E.coli

Tag

N-His

Sequence

Ser 618-Leu 824

Field of Research

Signal Transduction; Neuroscience; Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.7 kDa

Observed Molecular Weight

30 kDa

Species

Mouse