Recombinant Mouse FGA/Fibrinogen A protein (His tag)

Specifications

Catalog number

PDEM100293-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibrinogen is a 340 kDa, secreted glycoprotein complex that is found in blood at concentrations of 150-400 mg/dL. It is secreted primarily by hepatocytes, but is also reported to be expressed by fibroblasts, type I alveolar epithelium, intestinal epithelium and some tumor cells. Fibrinogen is a homodimer that is composed of two, three-polypeptide chain subunits. Fibrinogen plays a central role in clot formation. Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon- (gamma-glutamyl) lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrinogen is also a component of the ECM and binds to cell surface molecules on inflammatory cells. Mature mouse alpha, beta and gamma-chains share 67%, 85% and 83% aa identity with human alpha, beta and gamma-chains, respectively.

Abbreviation

FGA; Fibrinogen A

Synonyms

A alpha polypeptide; FGA; FPA; Fib2; Fibrinogen A; Fibrinogen Type I; Fibrinogen alpha chain; fibrinogen

UniProt

E9PV24

Accession Number

E9PV24

Expression System

E.coli

Tag

N-His

Sequence

Asp 434-Gln 789

Field of Research

Cardiovascular; Immunology; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.1 kDa

Observed Molecular Weight

50 kDa

Species

Mouse