Recombinant Mouse PSMA / FOLH1 protein (His tag)

Specifications

Catalog number

PDEM100301-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glutamate carboxypeptidase 2, also known as Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Prostate-specific membrane antigen, GCPII, PSMA, FOLH1, and NAALAD1, is a single-pass type I&nbsp, I membrane protein which belongs to the&nbsp, peptidase M28 family and M28B subfamily. FOLH1 is highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, brain (at protein level), and the capillary endothelium of a variety of tumors. FOLH1 has both folate hydrolase and N-acetylated alpha linked acidic dipeptidase (NAALADase) activity. It has a preference for tri-alpha-glutamate peptides. Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits. FOLH1 also shows a promising role in directed imaging and therapy of recurrent or metastatic disease.

Abbreviation

PSMA; FOLH1

Synonyms

Folate hydrolase 1; Folh1; Folylpoly-gamma-glutamate carboxypeptidase (FGCP) ; Glutamate carboxypeptidase 2; Glutamate carboxypeptidase II (GCPII) ; Membrane glutamate carboxypeptidase (mGCP) ; N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I)

UniProt

O35409

Accession Number

O35409

Expression System

E.coli

Tag

N-His

Sequence

Lys 45-Phe 335

Field of Research

Cancer; Metabolism; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

31.9 kDa

Observed Molecular Weight

38 kDa

Species

Mouse