Recombinant mouse IGF1/IGF‑I/IGF-1 protein (His tag)

Specifications

Catalog number

PDEM100305-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nsulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N-and C-terminal propeptides. These isoforms are differentially expressed by various tissues. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively, and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II. IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally. It plays an important role in muscle regeneration and tumor progression. IGF-I binds IGF-I R, IGF-II R, and the insulin receptor. IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors.

Abbreviation

IGF1; IGF-1

Synonyms

IGF-1; IGF1; Insulin-like growth factor I; Somatomedin C; insulin-like growth factor 1; somatomedin-C

UniProt

P05017

Accession Number

P05017

Expression System

E.coli

Tag

N-His

Sequence

Ala 48-Met 153

Field of Research

Cancer; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

11.6 kDa

Observed Molecular Weight

14 kDa

Species

Mouse