Recombinant Mouse CD22/Siglec-2 protein (His tag)

Specifications

Catalog number

PDEM100309-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6-linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

Abbreviation

CD22; Siglec-2

Synonyms

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22; CD22 antigenMGC130020; CD22 molecule; SIGLEC2FLJ22814; Siglec-2; T-cell surface antigen Leu-14; sialic acid binding Ig-like lectin 2

UniProt

P35329

Accession Number

P35329

Expression System

E.coli

Tag

N-His

Sequence

Ser 22-Thr 341

Field of Research

Cancer; Immunology; Stem Cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.1 kDa

Observed Molecular Weight

40 kDa

Species

Mouse