Recombinant Mouse CD20/MS4A1 protein (His tag)

Specifications

Catalog number

PDEM100311-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Abbreviation

CD20; MS4A1

Synonyms

B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; CD20; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; Ms4a1

UniProt

P19437

Accession Number

P19437

Expression System

E.coli

Tag

N-His

Sequence

Leu 133-Pro 291

Field of Research

Cancer; Immunolegy; Neuroscience; Stem Cells; Epigenetics and Nuclear signaling.

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.4 kDa

Observed Molecular Weight

28 kDa

Species

Mouse