Recombinant Mouse NGF/NGFB/beta-NGF protein (His tag)

Specifications

Catalog number

PDEM100312-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) . These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta-NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta-NGF and 95.8% with rat beta-NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Abbreviation

NGF; NGFB; beta-NGF

Synonyms

Beta-NGF; Beta-Nerve Growth Factor; NGF; NGFB

UniProt

P01139

Accession Number

P01139

Expression System

E.coli

Tag

N-His

Sequence

Ser 122-Gly 241

Field of Research

Cancer; Cell Biology; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.1 kDa

Observed Molecular Weight

15 kDa

Species

Mouse