Recombinant Mouse MUC16 protein (His tag)

Specifications

Catalog number

PDEM100315-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mucin-16 is a heavily O-glycosylated protein that can be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus, which induces proteolytic cleavage and liberation of extracellular domain. I provides protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Mucin-16 is composed of three domains: a Serine, Threonine-rich N-terminal domain, a repeated domain containing between 12 and 60 partially conserved tandem repeats of 156 amino acids and a C-terminal transmembrane domain with a short cytoplasmic tail.

Abbreviation

MUC16

Synonyms

Muc16; Mucin 16

UniProt

A0A140LJ72

Accession Number

A0A140LJ72

Expression System

E.coli

Tag

N-His

Sequence

Ile 6188-Ser 6372

Field of Research

Cancer; signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.2 kDa

Observed Molecular Weight

25 kDa

Species

Mouse